Journal: International Journal of Medical Sciences

Article Title: CD44+/CD105+ Human Amniotic Fluid Mesenchymal Stem Cells Survive and Proliferate in the Ovary Long-Term in a Mouse Model of Chemotherapy-Induced Premature Ovarian Failure

doi: 10.7150/ijms.4841

Figure Lengend Snippet: Analysis of the proliferation and survival of CD44+/CD105+ HuAFCs in vitro . (A) MTT assays indicated that the viability of CD44-/CD105- HuAFCs significantly reduced at both 2 d and 5 d, compared to CD44+/CD105+ HuAFCs. ** P < 0.01 vs. CD44-/CD105- HuAFCs; * P < 0.05 vs. CD44-/CD105- HuAFCs; # P > 0.05 vs. CD44-/CD105- HuAFCs; n = 3. (B) qRT-PCR analysis of Ki67 and survivin mRNA expression in CD44+/CD105+ and CD44-/CD105- HuAFCs; * P < 0.05 vs. CD44-/CD105- HuAFCs; # P > 0.05 vs. CD44-/CD105- HuAFCs; n = 3. (C) Western blotting analysis of Ki67 and survivin protein expression in CD44+/CD105+ and CD44-/CD105- HuAFCs; ** P < 0.01 vs. CD44+/CD105+ HuAFCs; * P < 0.05 vs. CD44+/CD105+ HuAFCs; # P > 0.05 vs. CD44+/CD105+ HuAFCs; n = 3. (D) Flow cytometric cell cycle analysis of CD44+/CD105+ and CD44-/CD105- HuAFCs. The majority of CD44-/CD105- HuAFCs were arrested in the G2/M phase with a reduced percentage of S phase cells; ** P < 0.01 vs. CD44+/CD105+ HuAFCs; * P < 0.05 vs. CD44+/CD105+ HuAFCs; # P > 0.05 vs. CD44+/CD105+ HuAFCs; n = 3. (E) FCM analysis of human mesenchymal stem cell marker expression in CD44+/CD105+ and CD44-/CD105- HuAFCs in vitro. Expression of the "stemness" markers was higher in CD44+/CD105+ HuAFCs than CD44-/CD105- HuAFCs.

Article Snippet: After blocking with 5% (w/v) non-fat dried milk in TBST (Tris-buffered saline containing Tween-20) (25 mM Tris/HCl, pH 8.0, 125 mM NaCl and 0.05% Tween-20), the PVDF membranes were washed 4 times (15 min each) with TBST at room temperature and incubated with primary antibody (rabbit anti-human Ki67 polyclonal antibody (1:1000; Chemicon, Temecula, CA, USA) and rabbit anti-human Survivin polyclonal antibody (1:1000; Chemicon, Temecula, CA, USA)).

Techniques: In Vitro, Quantitative RT-PCR, Expressing, Western Blot, Cell Cycle Assay, Marker

Journal: International Journal of Molecular Sciences

Article Title: Arachidin-1, a Prenylated Stilbenoid from Peanut, Induces Apoptosis in Triple-Negative Breast Cancer Cells

doi: 10.3390/ijms23031139

Figure Lengend Snippet: Western blot analysis and densitometry measurements of treated TNBC cell lines with arachidin-1 (A-1, 0.1, and 1 µM). Western blot images of ( A ) PARP and cleaved PARP, GAPDH loading control. ( B ) Full-length caspase-8, GAPDH loading control; ( C ) cleaved caspase-9, vinculin loading control; and ( D ) survivin, GAPDH loading control. Lysates (60 µg of total protein) of MDA-MB-231 and MDA-MB-436 cells treated with A-1 were analyzed by western blotting. The relative densitometry of ( E ) full-length PARP, ( F ) cleaved PARP, ( G ) caspase-8, ( H ) cleaved caspase-9, and ( I ) survivin protein levels were analyzed by comparing to the loading control. Cells with 0.01% DMSO were used as controls. Data represents mean ± SD from three or more independent experiments. * p < 0.05, ** p < 0.001, **** p < 0.0001 versus control in MDA-MB-231, # p < 0.05, ## p < 0.001, #### p < 0.00001 versus control in MDA-MB-436, and ns = not significant from control.

Article Snippet: The primary antibodies and dilutions included rabbit anti-caspase-8 (D35G2) mAb (1:500; Cell Signaling Technology; Danvers, MA, USA), rabbit anti-PARP mAb (1:500; Cell Signaling Technology; Danvers, MA, USA), rabbit anti-human survivin polyclonal antibody (0.5 μg/mL; R&D Systems; Minneapolis, MN, USA), mouse anti-human caspase-9 mAb (1 μg/mL; R&D Systems; Minneapolis, MN, USA), mouse anti-vinculin antibody (2 μg/mL; R&D Systems; Minneapolis, MN, USA), and mouse anti-human GAPDH antibody (0.05 μg/mL; R&D Systems; Minneapolis, MN, USA).

Techniques: Western Blot, Control

Journal: Oncology Letters

Article Title: Expression and clinical correlation of Survivin and PTEN in gastric cancer patients

doi: 10.3892/ol.2020.12160

Figure Lengend Snippet: Survivin and PTEN expression in gastric cancer and normal tissues.

Article Snippet: The slides were treated with rabbit polyclonal anti-human Survivin (cat. no. RAB-0536; NeoMarkers, Inc.) antibody (1:100) (ready to use) or mouse monoclonal anti-human PTEN (cat. no. 17A; NeoMarkers, Inc.) antibody (ready to use, 1:50) at room temperature for 2 h, followed by incubation with biotinylated goat anti-rabbit IgG (HRP; 1:10,000; cat. no. ab6721) and streptavidin-peroxidase (cat. no. TS-060-HR; Thermo Fisher Scientific, Inc.) for 1 h at room temperature.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Expression and clinical correlation of Survivin and PTEN in gastric cancer patients

doi: 10.3892/ol.2020.12160

Figure Lengend Snippet: Survivin expression. (A) GC tissue, (B) Adjacent normal tissue and (C) metastatic lymph node. The staining level demonstrated gradual increase in the sequence of GC tissue, adjacent normal tissue and metastatic lymph node. GC, gastric cancer.

Article Snippet: The slides were treated with rabbit polyclonal anti-human Survivin (cat. no. RAB-0536; NeoMarkers, Inc.) antibody (1:100) (ready to use) or mouse monoclonal anti-human PTEN (cat. no. 17A; NeoMarkers, Inc.) antibody (ready to use, 1:50) at room temperature for 2 h, followed by incubation with biotinylated goat anti-rabbit IgG (HRP; 1:10,000; cat. no. ab6721) and streptavidin-peroxidase (cat. no. TS-060-HR; Thermo Fisher Scientific, Inc.) for 1 h at room temperature.

Techniques: Expressing, Staining, Sequencing

Journal: Oncology Letters

Article Title: Expression and clinical correlation of Survivin and PTEN in gastric cancer patients

doi: 10.3892/ol.2020.12160

Figure Lengend Snippet: The correlation between Survivin and PTEN expression and clinicopathological factors.

Article Snippet: The slides were treated with rabbit polyclonal anti-human Survivin (cat. no. RAB-0536; NeoMarkers, Inc.) antibody (1:100) (ready to use) or mouse monoclonal anti-human PTEN (cat. no. 17A; NeoMarkers, Inc.) antibody (ready to use, 1:50) at room temperature for 2 h, followed by incubation with biotinylated goat anti-rabbit IgG (HRP; 1:10,000; cat. no. ab6721) and streptavidin-peroxidase (cat. no. TS-060-HR; Thermo Fisher Scientific, Inc.) for 1 h at room temperature.

Techniques: Expressing, Significance Assay

Journal: Oncology Letters

Article Title: Expression and clinical correlation of Survivin and PTEN in gastric cancer patients

doi: 10.3892/ol.2020.12160

Figure Lengend Snippet: Correlation between Survivin or PTEN expression and related factors of prognosis.

Article Snippet: The slides were treated with rabbit polyclonal anti-human Survivin (cat. no. RAB-0536; NeoMarkers, Inc.) antibody (1:100) (ready to use) or mouse monoclonal anti-human PTEN (cat. no. 17A; NeoMarkers, Inc.) antibody (ready to use, 1:50) at room temperature for 2 h, followed by incubation with biotinylated goat anti-rabbit IgG (HRP; 1:10,000; cat. no. ab6721) and streptavidin-peroxidase (cat. no. TS-060-HR; Thermo Fisher Scientific, Inc.) for 1 h at room temperature.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Expression and clinical correlation of Survivin and PTEN in gastric cancer patients

doi: 10.3892/ol.2020.12160

Figure Lengend Snippet: Relation between Survivin and PTEN expression.

Article Snippet: The slides were treated with rabbit polyclonal anti-human Survivin (cat. no. RAB-0536; NeoMarkers, Inc.) antibody (1:100) (ready to use) or mouse monoclonal anti-human PTEN (cat. no. 17A; NeoMarkers, Inc.) antibody (ready to use, 1:50) at room temperature for 2 h, followed by incubation with biotinylated goat anti-rabbit IgG (HRP; 1:10,000; cat. no. ab6721) and streptavidin-peroxidase (cat. no. TS-060-HR; Thermo Fisher Scientific, Inc.) for 1 h at room temperature.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Expression and clinical correlation of Survivin and PTEN in gastric cancer patients

doi: 10.3892/ol.2020.12160

Figure Lengend Snippet: Correlation between expression level of Survivin or PTEN in GC and survival. (A) The difference in overall survival between Survivin or PTEN positive and negative patients. (B) The difference in disease free survival between Survivin or PTEN positive and negative patients. (C) The difference in overall survival between patients with simultaneous Survivin + , PTEN − and patients with others (patients with Survivin+and PTEN+ or Survivin- and PTEN- or Survivin- and PTEN+). GC, gastric cancer; PTEN, phosphatase and tensin homolog.

Article Snippet: The slides were treated with rabbit polyclonal anti-human Survivin (cat. no. RAB-0536; NeoMarkers, Inc.) antibody (1:100) (ready to use) or mouse monoclonal anti-human PTEN (cat. no. 17A; NeoMarkers, Inc.) antibody (ready to use, 1:50) at room temperature for 2 h, followed by incubation with biotinylated goat anti-rabbit IgG (HRP; 1:10,000; cat. no. ab6721) and streptavidin-peroxidase (cat. no. TS-060-HR; Thermo Fisher Scientific, Inc.) for 1 h at room temperature.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Expression and clinical correlation of Survivin and PTEN in gastric cancer patients

doi: 10.3892/ol.2020.12160

Figure Lengend Snippet: Cox regression model for univariate and multivariate analyses of prognostic factors in gastric cancer.

Article Snippet: The slides were treated with rabbit polyclonal anti-human Survivin (cat. no. RAB-0536; NeoMarkers, Inc.) antibody (1:100) (ready to use) or mouse monoclonal anti-human PTEN (cat. no. 17A; NeoMarkers, Inc.) antibody (ready to use, 1:50) at room temperature for 2 h, followed by incubation with biotinylated goat anti-rabbit IgG (HRP; 1:10,000; cat. no. ab6721) and streptavidin-peroxidase (cat. no. TS-060-HR; Thermo Fisher Scientific, Inc.) for 1 h at room temperature.

Techniques: